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pw213  (Addgene inc)


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    Addgene inc pw213
    Pw213, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pw213/ZE40+pCS2P%2B+dkk1GFP+(Plasmid+%2317081)/pm34133940-510-46-43
    Average 93 stars, based on 4 article reviews
    pw213 - by Bioz Stars, 2026-09
    93/100 stars

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    Plasmid Preparation:

    Article Title: Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion.
    Article Snippet: 12145626 scRNA-seq dataset This paper GEO: GSE159780 Experimental models: Cell lines 293T Takara 632273 DLD-1 (parental) ATCC CCL-221 Dia-C6 (DLD-1 expressing 4 transgenes for inducible transcriptional modulation) This paper N/A D188 (Dia-C6 with lenti-sg-Control and NLS-mScarlet-I) This paper N/A D193 (Dia-C6 with lenti-sg-Control and NLS-mNeonGreen) This paper N/A D266 (Dia-C6 with lenti-sg2-Cdh1 andNLSmScarlet-I) This paper N/A D267 (Dia-C6 with lenti-sg1-Cdh1 andNLSmScarlet-I) This paper N/A D301 (Dia-C6 with lenti-sg-Itgb1 and NLSmNeonGreen) This paper N/A D2 (Dia-C6 with lenti-sg1-Cdh1, NLSmScarlet-I, lenti-sg-Itgb1 and NLSmNeonGreen) This paper N/A Experimental models: Organisms/strains Mouse: mTmG/HisG; FVB/N(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo Tg(HIST1H2BB-EGFP)1Pa/J Huebner et al., 2014 N/A Mouse: K14RFP/HisG; FVB/N(Cg)Tg(KRT14-RFP)#Efu Tg(HIST1H2BBEGFP)1Pa/J This paper N/A Mouse: KikGR; FVB/N(Cg)-Tg(KikGR)/N Hsu et al., 2013 N/A Mouse: ICR (CD-1) outbred mice Envigo Hsd:ICR(CD-1) Recombinant DNA Plasmid: Super piggyBac Transposase expression vector System Biosciences PB210PA-1 (Continued on next page) ll OPEN ACCESS e2 Cell 184, 3702–3716.e1–e17, July 8, 2021 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: pSLQ2818 (PiggyBac-ABACRISPRi) Gao et al., 2016 Addgene: 84241 Plasmid: pMD2.G Addgene Addgene: 12259 Plasmid: psPAX2 Addgene Addgene: 12260 Plasmid: pW210 (pPB-GA-inducibleCRISPRa-saCas9-VPR-hygR) This paper Addgene: 170808 Plasmid: pW211 (lenti-spsg-pEF1s-NLSmNeonGreen-P2A-BlastR) This paper Addgene: 170809 Plasmid: pW212 (lenti-spsg-pEF1s-NLSmScarlet-I-P2A-BlastR) This paper Addgene: 170810 Plasmid: pW213 (lenti-sasg-pEF1s-NLSmNeonGreen-P2A-BlastR) This paper Addgene: 170811 Plasmid: pW214 (lenti-sasg-pEF1s-NLSmScarlet-I-P2A-BlastR) This paper Addgene: 170812 Plasmid: pW188 (lenti-spsgRNA-lacZpEF1s-NLS-mScarlet-I-P2A-BlastR) This paper Addgene: 170813 Plasmid: pW193 (lenti-sasgRNA-lacZpEF1s-NLS-mNeonGreen-P2A-BlastR) This paper Addgene: 170814 Plasmid: pW266 (lenti-spsgRNA-sg2hsCDH1-pEF1s-NLS-mScarlet-I-P2ABlastR) This paper Addgene: 170815 Plasmid: pW267 (lenti-spsgRNA-sg1hsCDH1-pEF1s-NLS-mScarlet-I-P2ABlastR) This paper Addgene: 170816 Plasmid: pW301 (lenti-spsgRNA-hsITGB1sp-160rev-pEF1s-NLS-mNeonGreen-P2ABlastR) This paper Addgene: 170817 Software and algorithms ImageJ (Fiji) Schindelin et al., 2012 https://fiji.sc Imaris 9.5.0 Bitplane https://imaris.oxinst.com TrackMate Tinevez et al., 2017 https://imagej.net/TrackMate MorphoLibJ Legland et al., 2016 https://imagej.net/MorphoLibJ cellpose Stringer et al., 2021 http://www.cellpose.org/ Cell Ranger 10x Genomics https://support.10xgenomics.com/ single-cell-gene-expression/software/ pipelines/latest/what-is-cell-ranger Scanpy Wolf et al., 2018 https://scanpy.readthedocs.io/en/stable/ Velocyto La Manno et al., 2018 http://velocyto.org/velocyto.py/index.html scVelo Bergen et al., 2020 https://scvelo.readthedocs.io/ CRISPOR Haeussler et al., 2016 http://crispor.tefor.net smFISH quantification macros Wang, 2019 N/A Other Matrigel, Growth Factor Reduced Basement Membrane Matrix Corning 356231 Laminin I, Cultrex 3D Culture Matrix (contains low amount of collagen IV) Trevigen 3446-005-01 Accutase Innovative Cell Technologies AT104 Dispase II Thermo Fisher 17105041 Collagenase Elastin Products Company CL103 13mmdiameter 0.1 mmpore polycarbonate filters (for organ culture) MilliporeSigma WHA110405 (Continued on next page) ll OPEN ACCESS Cell 184, 3702–3716.e1–e17, July 8, 2021 e3 Article ..

    Software:

    Article Title: Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion.
    Article Snippet: 12145626 scRNA-seq dataset This paper GEO: GSE159780 Experimental models: Cell lines 293T Takara 632273 DLD-1 (parental) ATCC CCL-221 Dia-C6 (DLD-1 expressing 4 transgenes for inducible transcriptional modulation) This paper N/A D188 (Dia-C6 with lenti-sg-Control and NLS-mScarlet-I) This paper N/A D193 (Dia-C6 with lenti-sg-Control and NLS-mNeonGreen) This paper N/A D266 (Dia-C6 with lenti-sg2-Cdh1 andNLSmScarlet-I) This paper N/A D267 (Dia-C6 with lenti-sg1-Cdh1 andNLSmScarlet-I) This paper N/A D301 (Dia-C6 with lenti-sg-Itgb1 and NLSmNeonGreen) This paper N/A D2 (Dia-C6 with lenti-sg1-Cdh1, NLSmScarlet-I, lenti-sg-Itgb1 and NLSmNeonGreen) This paper N/A Experimental models: Organisms/strains Mouse: mTmG/HisG; FVB/N(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo Tg(HIST1H2BB-EGFP)1Pa/J Huebner et al., 2014 N/A Mouse: K14RFP/HisG; FVB/N(Cg)Tg(KRT14-RFP)#Efu Tg(HIST1H2BBEGFP)1Pa/J This paper N/A Mouse: KikGR; FVB/N(Cg)-Tg(KikGR)/N Hsu et al., 2013 N/A Mouse: ICR (CD-1) outbred mice Envigo Hsd:ICR(CD-1) Recombinant DNA Plasmid: Super piggyBac Transposase expression vector System Biosciences PB210PA-1 (Continued on next page) ll OPEN ACCESS e2 Cell 184, 3702–3716.e1–e17, July 8, 2021 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: pSLQ2818 (PiggyBac-ABACRISPRi) Gao et al., 2016 Addgene: 84241 Plasmid: pMD2.G Addgene Addgene: 12259 Plasmid: psPAX2 Addgene Addgene: 12260 Plasmid: pW210 (pPB-GA-inducibleCRISPRa-saCas9-VPR-hygR) This paper Addgene: 170808 Plasmid: pW211 (lenti-spsg-pEF1s-NLSmNeonGreen-P2A-BlastR) This paper Addgene: 170809 Plasmid: pW212 (lenti-spsg-pEF1s-NLSmScarlet-I-P2A-BlastR) This paper Addgene: 170810 Plasmid: pW213 (lenti-sasg-pEF1s-NLSmNeonGreen-P2A-BlastR) This paper Addgene: 170811 Plasmid: pW214 (lenti-sasg-pEF1s-NLSmScarlet-I-P2A-BlastR) This paper Addgene: 170812 Plasmid: pW188 (lenti-spsgRNA-lacZpEF1s-NLS-mScarlet-I-P2A-BlastR) This paper Addgene: 170813 Plasmid: pW193 (lenti-sasgRNA-lacZpEF1s-NLS-mNeonGreen-P2A-BlastR) This paper Addgene: 170814 Plasmid: pW266 (lenti-spsgRNA-sg2hsCDH1-pEF1s-NLS-mScarlet-I-P2ABlastR) This paper Addgene: 170815 Plasmid: pW267 (lenti-spsgRNA-sg1hsCDH1-pEF1s-NLS-mScarlet-I-P2ABlastR) This paper Addgene: 170816 Plasmid: pW301 (lenti-spsgRNA-hsITGB1sp-160rev-pEF1s-NLS-mNeonGreen-P2ABlastR) This paper Addgene: 170817 Software and algorithms ImageJ (Fiji) Schindelin et al., 2012 https://fiji.sc Imaris 9.5.0 Bitplane https://imaris.oxinst.com TrackMate Tinevez et al., 2017 https://imagej.net/TrackMate MorphoLibJ Legland et al., 2016 https://imagej.net/MorphoLibJ cellpose Stringer et al., 2021 http://www.cellpose.org/ Cell Ranger 10x Genomics https://support.10xgenomics.com/ single-cell-gene-expression/software/ pipelines/latest/what-is-cell-ranger Scanpy Wolf et al., 2018 https://scanpy.readthedocs.io/en/stable/ Velocyto La Manno et al., 2018 http://velocyto.org/velocyto.py/index.html scVelo Bergen et al., 2020 https://scvelo.readthedocs.io/ CRISPOR Haeussler et al., 2016 http://crispor.tefor.net smFISH quantification macros Wang, 2019 N/A Other Matrigel, Growth Factor Reduced Basement Membrane Matrix Corning 356231 Laminin I, Cultrex 3D Culture Matrix (contains low amount of collagen IV) Trevigen 3446-005-01 Accutase Innovative Cell Technologies AT104 Dispase II Thermo Fisher 17105041 Collagenase Elastin Products Company CL103 13mmdiameter 0.1 mmpore polycarbonate filters (for organ culture) MilliporeSigma WHA110405 (Continued on next page) ll OPEN ACCESS Cell 184, 3702–3716.e1–e17, July 8, 2021 e3 Article ..

    Membrane:

    Article Title: Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion.
    Article Snippet: 12145626 scRNA-seq dataset This paper GEO: GSE159780 Experimental models: Cell lines 293T Takara 632273 DLD-1 (parental) ATCC CCL-221 Dia-C6 (DLD-1 expressing 4 transgenes for inducible transcriptional modulation) This paper N/A D188 (Dia-C6 with lenti-sg-Control and NLS-mScarlet-I) This paper N/A D193 (Dia-C6 with lenti-sg-Control and NLS-mNeonGreen) This paper N/A D266 (Dia-C6 with lenti-sg2-Cdh1 andNLSmScarlet-I) This paper N/A D267 (Dia-C6 with lenti-sg1-Cdh1 andNLSmScarlet-I) This paper N/A D301 (Dia-C6 with lenti-sg-Itgb1 and NLSmNeonGreen) This paper N/A D2 (Dia-C6 with lenti-sg1-Cdh1, NLSmScarlet-I, lenti-sg-Itgb1 and NLSmNeonGreen) This paper N/A Experimental models: Organisms/strains Mouse: mTmG/HisG; FVB/N(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo Tg(HIST1H2BB-EGFP)1Pa/J Huebner et al., 2014 N/A Mouse: K14RFP/HisG; FVB/N(Cg)Tg(KRT14-RFP)#Efu Tg(HIST1H2BBEGFP)1Pa/J This paper N/A Mouse: KikGR; FVB/N(Cg)-Tg(KikGR)/N Hsu et al., 2013 N/A Mouse: ICR (CD-1) outbred mice Envigo Hsd:ICR(CD-1) Recombinant DNA Plasmid: Super piggyBac Transposase expression vector System Biosciences PB210PA-1 (Continued on next page) ll OPEN ACCESS e2 Cell 184, 3702–3716.e1–e17, July 8, 2021 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: pSLQ2818 (PiggyBac-ABACRISPRi) Gao et al., 2016 Addgene: 84241 Plasmid: pMD2.G Addgene Addgene: 12259 Plasmid: psPAX2 Addgene Addgene: 12260 Plasmid: pW210 (pPB-GA-inducibleCRISPRa-saCas9-VPR-hygR) This paper Addgene: 170808 Plasmid: pW211 (lenti-spsg-pEF1s-NLSmNeonGreen-P2A-BlastR) This paper Addgene: 170809 Plasmid: pW212 (lenti-spsg-pEF1s-NLSmScarlet-I-P2A-BlastR) This paper Addgene: 170810 Plasmid: pW213 (lenti-sasg-pEF1s-NLSmNeonGreen-P2A-BlastR) This paper Addgene: 170811 Plasmid: pW214 (lenti-sasg-pEF1s-NLSmScarlet-I-P2A-BlastR) This paper Addgene: 170812 Plasmid: pW188 (lenti-spsgRNA-lacZpEF1s-NLS-mScarlet-I-P2A-BlastR) This paper Addgene: 170813 Plasmid: pW193 (lenti-sasgRNA-lacZpEF1s-NLS-mNeonGreen-P2A-BlastR) This paper Addgene: 170814 Plasmid: pW266 (lenti-spsgRNA-sg2hsCDH1-pEF1s-NLS-mScarlet-I-P2ABlastR) This paper Addgene: 170815 Plasmid: pW267 (lenti-spsgRNA-sg1hsCDH1-pEF1s-NLS-mScarlet-I-P2ABlastR) This paper Addgene: 170816 Plasmid: pW301 (lenti-spsgRNA-hsITGB1sp-160rev-pEF1s-NLS-mNeonGreen-P2ABlastR) This paper Addgene: 170817 Software and algorithms ImageJ (Fiji) Schindelin et al., 2012 https://fiji.sc Imaris 9.5.0 Bitplane https://imaris.oxinst.com TrackMate Tinevez et al., 2017 https://imagej.net/TrackMate MorphoLibJ Legland et al., 2016 https://imagej.net/MorphoLibJ cellpose Stringer et al., 2021 http://www.cellpose.org/ Cell Ranger 10x Genomics https://support.10xgenomics.com/ single-cell-gene-expression/software/ pipelines/latest/what-is-cell-ranger Scanpy Wolf et al., 2018 https://scanpy.readthedocs.io/en/stable/ Velocyto La Manno et al., 2018 http://velocyto.org/velocyto.py/index.html scVelo Bergen et al., 2020 https://scvelo.readthedocs.io/ CRISPOR Haeussler et al., 2016 http://crispor.tefor.net smFISH quantification macros Wang, 2019 N/A Other Matrigel, Growth Factor Reduced Basement Membrane Matrix Corning 356231 Laminin I, Cultrex 3D Culture Matrix (contains low amount of collagen IV) Trevigen 3446-005-01 Accutase Innovative Cell Technologies AT104 Dispase II Thermo Fisher 17105041 Collagenase Elastin Products Company CL103 13mmdiameter 0.1 mmpore polycarbonate filters (for organ culture) MilliporeSigma WHA110405 (Continued on next page) ll OPEN ACCESS Cell 184, 3702–3716.e1–e17, July 8, 2021 e3 Article ..

    Organ Culture:

    Article Title: Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion.
    Article Snippet: 12145626 scRNA-seq dataset This paper GEO: GSE159780 Experimental models: Cell lines 293T Takara 632273 DLD-1 (parental) ATCC CCL-221 Dia-C6 (DLD-1 expressing 4 transgenes for inducible transcriptional modulation) This paper N/A D188 (Dia-C6 with lenti-sg-Control and NLS-mScarlet-I) This paper N/A D193 (Dia-C6 with lenti-sg-Control and NLS-mNeonGreen) This paper N/A D266 (Dia-C6 with lenti-sg2-Cdh1 andNLSmScarlet-I) This paper N/A D267 (Dia-C6 with lenti-sg1-Cdh1 andNLSmScarlet-I) This paper N/A D301 (Dia-C6 with lenti-sg-Itgb1 and NLSmNeonGreen) This paper N/A D2 (Dia-C6 with lenti-sg1-Cdh1, NLSmScarlet-I, lenti-sg-Itgb1 and NLSmNeonGreen) This paper N/A Experimental models: Organisms/strains Mouse: mTmG/HisG; FVB/N(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo Tg(HIST1H2BB-EGFP)1Pa/J Huebner et al., 2014 N/A Mouse: K14RFP/HisG; FVB/N(Cg)Tg(KRT14-RFP)#Efu Tg(HIST1H2BBEGFP)1Pa/J This paper N/A Mouse: KikGR; FVB/N(Cg)-Tg(KikGR)/N Hsu et al., 2013 N/A Mouse: ICR (CD-1) outbred mice Envigo Hsd:ICR(CD-1) Recombinant DNA Plasmid: Super piggyBac Transposase expression vector System Biosciences PB210PA-1 (Continued on next page) ll OPEN ACCESS e2 Cell 184, 3702–3716.e1–e17, July 8, 2021 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: pSLQ2818 (PiggyBac-ABACRISPRi) Gao et al., 2016 Addgene: 84241 Plasmid: pMD2.G Addgene Addgene: 12259 Plasmid: psPAX2 Addgene Addgene: 12260 Plasmid: pW210 (pPB-GA-inducibleCRISPRa-saCas9-VPR-hygR) This paper Addgene: 170808 Plasmid: pW211 (lenti-spsg-pEF1s-NLSmNeonGreen-P2A-BlastR) This paper Addgene: 170809 Plasmid: pW212 (lenti-spsg-pEF1s-NLSmScarlet-I-P2A-BlastR) This paper Addgene: 170810 Plasmid: pW213 (lenti-sasg-pEF1s-NLSmNeonGreen-P2A-BlastR) This paper Addgene: 170811 Plasmid: pW214 (lenti-sasg-pEF1s-NLSmScarlet-I-P2A-BlastR) This paper Addgene: 170812 Plasmid: pW188 (lenti-spsgRNA-lacZpEF1s-NLS-mScarlet-I-P2A-BlastR) This paper Addgene: 170813 Plasmid: pW193 (lenti-sasgRNA-lacZpEF1s-NLS-mNeonGreen-P2A-BlastR) This paper Addgene: 170814 Plasmid: pW266 (lenti-spsgRNA-sg2hsCDH1-pEF1s-NLS-mScarlet-I-P2ABlastR) This paper Addgene: 170815 Plasmid: pW267 (lenti-spsgRNA-sg1hsCDH1-pEF1s-NLS-mScarlet-I-P2ABlastR) This paper Addgene: 170816 Plasmid: pW301 (lenti-spsgRNA-hsITGB1sp-160rev-pEF1s-NLS-mNeonGreen-P2ABlastR) This paper Addgene: 170817 Software and algorithms ImageJ (Fiji) Schindelin et al., 2012 https://fiji.sc Imaris 9.5.0 Bitplane https://imaris.oxinst.com TrackMate Tinevez et al., 2017 https://imagej.net/TrackMate MorphoLibJ Legland et al., 2016 https://imagej.net/MorphoLibJ cellpose Stringer et al., 2021 http://www.cellpose.org/ Cell Ranger 10x Genomics https://support.10xgenomics.com/ single-cell-gene-expression/software/ pipelines/latest/what-is-cell-ranger Scanpy Wolf et al., 2018 https://scanpy.readthedocs.io/en/stable/ Velocyto La Manno et al., 2018 http://velocyto.org/velocyto.py/index.html scVelo Bergen et al., 2020 https://scvelo.readthedocs.io/ CRISPOR Haeussler et al., 2016 http://crispor.tefor.net smFISH quantification macros Wang, 2019 N/A Other Matrigel, Growth Factor Reduced Basement Membrane Matrix Corning 356231 Laminin I, Cultrex 3D Culture Matrix (contains low amount of collagen IV) Trevigen 3446-005-01 Accutase Innovative Cell Technologies AT104 Dispase II Thermo Fisher 17105041 Collagenase Elastin Products Company CL103 13mmdiameter 0.1 mmpore polycarbonate filters (for organ culture) MilliporeSigma WHA110405 (Continued on next page) ll OPEN ACCESS Cell 184, 3702–3716.e1–e17, July 8, 2021 e3 Article ..



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